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KMID : 0351619740150010279
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Kyungpook Medical Journal
1974 Volume.15 No. 1 p.279 ~ p.291
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The Changes of Liver Cell Fine Structure Induced by RNA Synthesis Inhibition
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Abstract
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This study was carried out to investigate the ultrastructural. changes of rat liver ells induced by administration of a protein synthesis reducing agent, either ethionine or actinomycin D al-?ne, or pretreatment with ethionine followed by actinomycin D.
The results were as follow:
Ethionine induced main effects on the cytoplasmic organelles such as dilatation of rER, proliferation and dilatation of rER, proliferation and dilatation of sER´, detachment of ribosome from the rER membrane, degeneration and loss of ribosome, and swelli~ig and loss of cristae of mitochondria. Severely damaged cells showed mild degree of nuclear change:; such as irregularity r)f nuclear magin and condensation of chromatin.
Actinomycin D induced main effects on the nucleolus, such as segregation of nucleolar substances into fibrillar and granular components and degenerative changes. Cytoplasmic organelles shows marked increase of sER with marked decrease of rER, shedding and loss of ribosome, and loss of cristae of mitochondria.
Both ethionine and actinomycin D treated group showed more marked changes of cytoplasmic organelles and nucleolus.
The results suggest that ethionine primarily effects on the cytoplasmi corganelles but actinomycin D primarily effects on the nucleolus. The changes of cytoplasmic organelles are not greatly different between two drugs, The nucleolar changes induced by actinomycin D may reflect deranged functicn of nucleolus and the degenerative changes of cytoplasmic organ~;lles may be induced by deranged function of enzyme system of the cells.
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